Introducing color into stacking gels makes sample loading easy.

0003-2697/$ see front matter 2007 Elsevier Inc. All rights reserved. doi:10.1016/j.ab.2007.04.002 * Corresponding author. E-mail address: [email protected] (Y. Ryabov). Fig. 1. Traditional (left half of the figure) and new (right half of the figure) rend gels before loading; bottom panels show the scans of the processed gels. I 4%-acrylamide stacking buffer (upper right). The performance of the gels has wells. This standard technology leads to one apparent inconvenience—the wells are barely visible in the colorless and transparent electrophoretic solution. As a result, loading the sample onto the gel is more difficult than it should be. We propose a simple improvement to the existing procedure whereupon the color is added to the stacking portion